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Antineoplastic Study of Volatile Oils from 12 Plants on Cultured Cancer Cells.
Ngamkham J*, Ondee S*, Reinkijakarn M*, Donchai A**, Kawaree R**, Phutdhawong W**, Picha P*
*Research Division, National Cancer Institute, Bangkok
**Department of Chemistry, Faculty of Science, Maejo University, ChiangMai
Abstract
The volatile oils from fresh leaves of 12 plants, Polyacias fruticosa Harms, Emilia sonchifolia DC, Polygonum odoratum Lour, Streblus asper Lour, Eryngium foetidum Linn, Houttuynia cordata Thunb, Cinnamomum bdjolghota Sweet, Anethum graveolens Linn, Knema Lenta Warb, Eupatorium odoraum Linn, Piper sarmentosum and Spilanthes acmella Murr, were isolated by hydrodistillation. These 12 volatile oil extracts were then tested for in vitro cytotoxicity on murine lymphocytic, P388; human mammary carcinoma, MCF-7 and human epidermoid carcinoma of floor of the mouth, KB. The cytotoxicity assays were undertaken with a dye exclusion method or by total cell protein determination and expressed with reference to the median effective dose (ED50), following the criteria of the U.S. National Cancer Institute. An ED50 below 30 mg/ml was set as the criteria for cytotoxicity. Our investigation, 9 of the 12 volatile oils to possess ED50 values more than 30 mg/ml with KB cells and 11 of the 12 volatile oils show ED50 values more than 30mg/ml with MCF-7 cells. In contrast, 6 of the 12 volatile oil extracts were remarkably cytotoxic towards the P388 cell line. In summary, the 6 volatile oil extracts from 12 plants proved more cytotoxic against P388 than MCF-7 and KB cells and showed promise for further studies of phyto-chemistry, bioassay-directed fractionation and anti-tumor activity in an animal tumor model
Keywords: antineoplastic, plant volatile, tumor cell culture, cytotoxicity test
ตีพิมพ์ในวารสารกรมการแพทย์ Volume 31, Number 3, Jul-Sep, 2006 หน้า 215-222
(Bull Dept Med Serv 2006; 31: 215-22)
