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Laboratory Animal in Cancer Chemopreventive Studies.
Piengchai Kupradinun 1*, Anong Tepsuwan 2, Pongpun Siripong 3,Danai Tivavech 4, and Wannee R. Kusamran 2
1* Laboratory Animal Section,
2 Biochemistry and Chemical Carcinogenesis Section,
3 National Products Research Section, 4 Molecular Biology Section,
Research Division, National Cancer Institute, Department of Medical Services,
Ministry of Public Health, Rama 6 Rd., Rajchthevi, Bangkok 10400, Thailand.
*Corresponding author: E-mail : pkupradi@health.moph.go.th
Abstract
Cancer has been the leading cause of death in Thailand since 2000. Chemoprevention is the new promising strategy for cancer prevention by the use of either synthetic or naturally occurring substances to intervene in the progression of carcinogenesis. At present, there are a large number of chemicals found to exhibit antimutagenic and anticarcinogenic effects in numerous animal and cell culture systems. Recent research in the Research Division of the National Cancer Institute, Thailand was on the evaluation of chemopreventive properties of some Thai dietary and medicinal plants using many type of studies in laboratory animal. In vivo erythrocyte micronucleus assay in the mouse or short term assay for carcinogenicity has been used for anticlastogenicity of Thai dietary and medicinal plants. Medium term test of carcinogenicity (Ito’s Liver Model) using preneoplastic glutathione S-tranferase placental form (GST-P) positive rat liver cell foci as marker lesions can be applied for screening chemoprotective potential of some herbs. Long term animal carcinogenicity studies i.e. liver, mammary gland or colon cancer models in rats and mice induced by chemical carcinogens also have been used to determine the chemopreventive properties of Thai dietary and medicinal plants. Hence, animal models are important in screening appropriate plants for preventive approaches of human cancer.
Key words: Animal, cancer model, chemoprevention, dietary and medicinal plants
Presented at The International Symposium on Laboratory Animal Science 2009 (ILAS2009) “Ethics, Welfare and Legislation on the Use and Care of Laboratory Animals” 26-29 April, 2009 at the Royal Thai Navy Convention Hall, Bangkok, Thailand.
